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YEAST[]

Yeast transformation stationary (LKL 2014 protocol)

Yeast Transformation High Efficiency old school - use this one

Yeast Transformation High Effeicieny newer

Yeast Transformation High Effeciency

Yeast integration overview

Yeast media

Mating / Sporulation / Tetrads

Genomic DNA isolation 'smash & grab'

Colony PCR

Yeast spotting assay

Yeast suppressor screen protocol (on this wiki)

setup for RLS[]

RLS dissection

CLS

Bioscreen

replica plating (link to Mark's wiki)

generate rho0 yeast

mtDNA loss

Other[]

PCR template

Western blot

BCA assay

Gateway cloning

Sequencing at UW


Drug stocks

Qiagen Kits[]

Miniprep

PCR cleanup

Gel Extraction


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PCR toolbox info from 2004 paper "A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes"

More protocols[]

find more protocols here-> - http://getyourscienceon.wikia.com/wiki/Protocols

and some here -> http://getyourscienceon.wikia.com/wiki/GDMC_protocols

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